pi cell cycle analysis kit Search Results


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Guangzhou JET Bio-Filtration annexin v-fitc/pi apoptosis kit
Annexin V Fitc/Pi Apoptosis Kit, supplied by Guangzhou JET Bio-Filtration, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson an annexin v/dead cell kit
An Annexin V/Dead Cell Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson propidium iodide (pi) staining cell cycle assay kit
Effect of EESB on the HT-29 cell cycle. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were stained with <t>propidium</t> iodide and analyzed by fluorescence-activated cell sorting. The proportion of DNA in the S phase was calculated using ModFit LT version 3.0 software. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6.
Propidium Iodide (Pi) Staining Cell Cycle Assay Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson pi/rnase staining buffer cell cycle assay kit
Effect of EESB on the HT-29 cell cycle. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were stained with <t>propidium</t> iodide and analyzed by fluorescence-activated cell sorting. The proportion of DNA in the S phase was calculated using ModFit LT version 3.0 software. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6.
Pi/Rnase Staining Buffer Cell Cycle Assay Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nexcelom Bioscience cellometer pi cell cycle kit
Effect of EESB on the HT-29 cell cycle. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were stained with <t>propidium</t> iodide and analyzed by fluorescence-activated cell sorting. The proportion of DNA in the S phase was calculated using ModFit LT version 3.0 software. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6.
Cellometer Pi Cell Cycle Kit, supplied by Nexcelom Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nexcelom Bioscience pi cell cycle kit
Effect of EESB on the HT-29 cell cycle. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were stained with <t>propidium</t> iodide and analyzed by fluorescence-activated cell sorting. The proportion of DNA in the S phase was calculated using ModFit LT version 3.0 software. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6.
Pi Cell Cycle Kit, supplied by Nexcelom Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson cell cycle staining kit pi/rnase staining buffer #550825
Effect of EESB on the HT-29 cell cycle. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were stained with <t>propidium</t> iodide and analyzed by fluorescence-activated cell sorting. The proportion of DNA in the S phase was calculated using ModFit LT version 3.0 software. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6.
Cell Cycle Staining Kit Pi/Rnase Staining Buffer #550825, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Guangzhou JET Bio-Filtration e-click edu cell proliferation imaging assay kit
Effect of EESB on the HT-29 cell cycle. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were stained with <t>propidium</t> iodide and analyzed by fluorescence-activated cell sorting. The proportion of DNA in the S phase was calculated using ModFit LT version 3.0 software. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6.
E Click Edu Cell Proliferation Imaging Assay Kit, supplied by Guangzhou JET Bio-Filtration, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson cell cycle staining (pi) kit
Effect of EESB on the HT-29 cell cycle. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were stained with <t>propidium</t> iodide and analyzed by fluorescence-activated cell sorting. The proportion of DNA in the S phase was calculated using ModFit LT version 3.0 software. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6.
Cell Cycle Staining (Pi) Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson cell cycle kit bd pharmingentm pi/rnase staining buffer
Effect of EESB on the HT-29 cell cycle. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were stained with <t>propidium</t> iodide and analyzed by fluorescence-activated cell sorting. The proportion of DNA in the S phase was calculated using ModFit LT version 3.0 software. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6.
Cell Cycle Kit Bd Pharmingentm Pi/Rnase Staining Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Canvax Inc pi cell cycle analysis kit
Effect of EESB on the HT-29 cell cycle. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were stained with <t>propidium</t> iodide and analyzed by fluorescence-activated cell sorting. The proportion of DNA in the S phase was calculated using ModFit LT version 3.0 software. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6.
Pi Cell Cycle Analysis Kit, supplied by Canvax Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of EESB on the HT-29 cell cycle. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were stained with propidium iodide and analyzed by fluorescence-activated cell sorting. The proportion of DNA in the S phase was calculated using ModFit LT version 3.0 software. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6.

Journal: Experimental and Therapeutic Medicine

Article Title: Scutellaria barbata D. Don inhibits growth and induces apoptosis by suppressing IL-6-inducible STAT3 pathway activation in human colorectal cancer cells

doi: 10.3892/etm.2015.2692

Figure Lengend Snippet: Effect of EESB on the HT-29 cell cycle. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were stained with propidium iodide and analyzed by fluorescence-activated cell sorting. The proportion of DNA in the S phase was calculated using ModFit LT version 3.0 software. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6.

Article Snippet: The HT-29 cell cycle progression was determined through flow cytometric analysis using a propidium iodide (PI) staining cell cycle assay kit (BD Biosciences, Franklin Lakes, NJ, USA).

Techniques: Concentration Assay, Staining, Fluorescence, FACS, Software, Standard Deviation

Effect of EESB on HT-29 cell apoptosis. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were collected and stained with Annexin V/PI, followed by fluorescence-activated cell sorting analysis. Double-negative stained cells indicate the live cell population; Annexin V-positive/PI-negative stained cells and Annexin V/PI double-positive stained cells represent early and late apoptosis, respectively; Annexin V-negative and PI-positive stained cells show dead cells. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6; UL, upper left; UR, upper right; LR, lower right; LL, lower left; PI, propidium iodide; FITC, fluorescein isothiocyanate.

Journal: Experimental and Therapeutic Medicine

Article Title: Scutellaria barbata D. Don inhibits growth and induces apoptosis by suppressing IL-6-inducible STAT3 pathway activation in human colorectal cancer cells

doi: 10.3892/etm.2015.2692

Figure Lengend Snippet: Effect of EESB on HT-29 cell apoptosis. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were collected and stained with Annexin V/PI, followed by fluorescence-activated cell sorting analysis. Double-negative stained cells indicate the live cell population; Annexin V-positive/PI-negative stained cells and Annexin V/PI double-positive stained cells represent early and late apoptosis, respectively; Annexin V-negative and PI-positive stained cells show dead cells. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6; UL, upper left; UR, upper right; LR, lower right; LL, lower left; PI, propidium iodide; FITC, fluorescein isothiocyanate.

Article Snippet: The HT-29 cell cycle progression was determined through flow cytometric analysis using a propidium iodide (PI) staining cell cycle assay kit (BD Biosciences, Franklin Lakes, NJ, USA).

Techniques: Concentration Assay, Staining, Fluorescence, FACS, Standard Deviation